effect of efflux pump inhibitor carbonyl cyanide 3-chlorophenylhydrazone on the minimum inhibitory concentration of ciprofloxacin in acinetobacter baumannii clinical isolates
نویسندگان
چکیده
background: acinetobacter baumannii is an important human pathogen with increasing notoriety in the recent years, as a causative organism of drug resistant nosocomial infections, particularly in immunocompromised patients hospitalized in burn centers. objectives: the aim of this study was to determinate the role of efflux pump(s) in ciprofloxacin resistance of a. baumannii strains isolated from burn patients. materials and methods: sixty-five a. baumannii strains were isolated from the burn patients hospitalized in motahari burns and reconstruction center in tehran, iran. susceptibility test to ciprofloxacin was carried out by disk agar diffusion and agar dilution methods, according to the clsi guidelines. activity of the efflux system was evaluated using efflux pump inhibitor carbonyl cyanide 3-chlorophenylhydrazone (cccp). results: all acinetobacter isolates were resistant to ciprofloxacin. the minimum inhibitory concentration (mic) range of ciprofloxacin in isolates was 4 to 128 µg/ml or greater. moreover, susceptibility of strains to ciprofloxacin was highly increased in the presence of efflux pump inhibitor; so that, for 86.1% (56/65) of isolates, cccp reduced the mic by 2 to 64 folds. conclusions: our findings are suggestive that efflux-based system may play a role in fluoroquinolone resistance in a. baumannii isolates, affecting hospitalized patients. the ability of acinetobacter to acquire resistance to these potent antimicrobials by the efflux pump mechanism is a concern. therefore, new strategies are required in order to eliminate the efflux transport activity from the resistant bacteria causing nosocomial infections and provide more appropriate approaches for treatment and management of troubling infections.
منابع مشابه
Effect of Efflux Pump Inhibitor Carbonyl Cyanide 3-Chlorophenylhydrazone on the Minimum Inhibitory Concentration of Ciprofloxacin in Acinetobacter baumannii Clinical Isolates
BACKGROUND Acinetobacter baumannii is an important human pathogen with increasing notoriety in the recent years, as a causative organism of drug resistant nosocomial infections, particularly in immunocompromised patients hospitalized in burn centers. OBJECTIVES The aim of this study was to determinate the role of efflux pump(s) in ciprofloxacin resistance of A. baumannii strains isolated from...
متن کاملDetection of Efflux Pump Using Ethidium Bromide-Agar Cartwheel Method in Acinetobacter baumannii Clinical Isolates
Introduction: In the past decade, multidrug-resistant Acinetobacter baumannii has become one of the most critical challenges in treating infected patients. The AdeABC efflux pump is the most important among the various resistance mechanisms. This pump can force various antibiotics and ethidium bromide out of the bacterial cell to the surrounding environment. Methods: In this study, nine A. baum...
متن کاملCorrelation of Ciprofloxacin Resistance with the AdeABC Efflux System in Acinetobacter baumannii Clinical Isolates
BACKGROUND Acinetobacter baumannii is one of the most important pathogens capable of colonization in burn patients, leading to drug-resistant wound infections. This study evaluated the distribution of the AdeABC efflux system genes and their relationship to ciprofloxacin resistance in A. baumannii isolates collected from burn patients. METHODS A total of 68 A. baumannii clinical strains were ...
متن کاملEvaluation of Phenotypic and Genotypic Expression of Efflux Pumpin Clinical Isolates of Acinetobacter baumannii Resistant to Ciprofloxacin and Gentamicin
Introduction: Acinetobacter baumannii is an important bacteria because of high ability to obtain antibiotic resistance genes and creation of multi-drug resistant strains (MDR). Today, the active efflux pumps has been suggested as one of the most important mechanisms of intrinsic and acquired resistance of antibiotics in bacteria. The aim of this study was Evaluation of phenotypic and genotypic ...
متن کاملadeabc efflux pump genes in multidrug resistant acinetobacter baumannii isolates
conclusions adeabc efflux system plays a vital role in multidrug resistance in clinical a. baumannii isolates. it was noted that the most important gene responsible for multidrug resistance within this system was the adeb gene especially in carbapenems resistance. methods this study was conducted on 84 multidrug resistant and 13 carbapenem-susceptible a. baumannii isolates obtained from burn an...
متن کاملفنوتیپ و ژنوتیپ پمپهای ایفلاکس در ایزولههای گونههای اسینتوباکتر جدا شده از بیماران بستری در بیمارستانهای امام رضا و طالقانی کرمانشاه در سال 1392
Background and purpose: Acinetobacter spp. is a non-fermentative gram- negative bacterium involving in various nosocomial infections. During last decade increasing number of multidrug-resistant isolates of Acinetobacter spp. have been reported. This type of resistance is possibly related to efflux pumps. This research aimed at identifying the phonotypic and genotypic characteristics of efflux p...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
jundishapur journal of microbiologyجلد ۷، شماره ۱، صفحات ۰-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023